Anti-Alpha-tubulin TUBA3C Antibody

Product Name

Anti-Alpha-tubulin TUBA3C Antibody

View all alpha Tubulin 3c Antibodies

SKU/Catalog Number

A17842-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Alpha-tubulin TUBA3C Antibody (Catalog # A17842-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Alpha-tubulin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Alpha-tubulin TUBA3C Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17842-1)

Host

Rabbit

Contents

Alpha-tubulin antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

Alpha-tubulin antibody was raised against an 18 amino acid peptide near the amino terminus of human alpha-tubulin. The immunogen is located within the first 50 amino acids of Alpha-tubulin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Alpha-tubulin antibody is human, mouse and rat reactive.

Reactive Species

A17842-1 is reactive to TUBA3C in Human, Mouse, Rat

Applications

A17842-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

49.96kDa

Background of alpha Tubulin 3c

Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Alpha-tubulin antibody can be used for detection of alpha-tubulin by Western blot at 0.25 - 0.5 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of alpha-tubulin in human brain tissue lysate with alpha-tubulin antibody at (A) 0.25 and (B) 0.5 μg/ml.

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Immunohistochemistry of Alpha-tubulin in human brain tissue with Alpha-tubulin antibody at 5 μg/mL.

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Immunofluorescence of Alpha-tubulin in human brain tissue with Alpha-tubulin antibody at 20 μg/mL.

Gene/Protein Information For TUBA3C (Source: Uniprot.org, NCBI)

Alternative Names

Alpha-tubulin 2; Alpha-tubulin 3C/D; bA408E5.3; TUBA2Tubulin alpha-2 chain; TUBA3D; tubulin alpha-3C/D chain; tubulin, alpha 2; tubulin, alpha 3c TUBA3C TUBA2, bA408E5.3 tubulin alpha 3c tubulin alpha-3C chain|Alpha-tubulin 3D|Tubulin alpha-3D chain|alpha-tubulin 2|alpha-tubulin 3C|alpha-tubulin 3C/D|tubulin alpha-2 chain|tubulin alpha-3C/D chain|tubulin, alpha 2

For more info on TUBA3C, check out the TUBA3C Infographic

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In this infographic, you will see the following information for TUBA3C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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